\n
FOIs reveal that health\/science institutions around the world (182 and counting!) have no record of SARS-COV-2 isolation\/purification, anywhere, ever<\/a><\/blockquote><\/iframe>\n<\/div><\/figure>\n\n\n\nKonfrontovala Steva ohledn\u011b jeho tvrzen\u00ed o “izolaci” a brilantn\u011b pouk\u00e1zala na to, pro\u010d se m\u00fdl\u00ed. M\u00edsto toho, aby uznal, \u017ee m\u00e1 pravdu a \u017ee t\u00e9matu zjevn\u011b nerozum\u00ed, Steve se ke sv\u00e9mu sm\u011b\u0161n\u00e9mu tvrzen\u00ed hrbil a tla\u010dil ji do p\u011btihodinov\u00e9 \u017eiv\u00e9 debaty se sv\u00fdmi “odborn\u00edky”, aby nechal div\u00e1ky rozhodnout, kter\u00e1 strana m\u00e1 v argumentu o izolaci “SARS-COV-2” pravdu. Bez ohledu na sm\u011b\u0161nost p\u011btihodinov\u00e9ho \u010dasov\u00e9ho r\u00e1mce a touhu div\u00e1k\u016f rozhodnout o v\u00edt\u011bzi se Steve pokou\u0161el sed\u011bt stranou a hr\u00e1t si na dohazova\u010de t\u00edm, \u017ee postavil sv\u00e9 “odborn\u00edky” proti Christine. Jakmile si Christine vzala na pomoc t\u00fdm sv\u00fdch vlastn\u00edch odborn\u00edk\u016f, Steve z\u0159ejm\u011b zpanika\u0159il a rozhodl se odej\u00edt z jevi\u0161t\u011b vlevo.<\/p>\n\n\n\nToto je jen stru\u010dn\u00e9 shrnut\u00ed toho, co se odehr\u00e1lo v pr\u016fb\u011bhu n\u011bkolika t\u00fddn\u016f v lednu 2022, a mo\u017en\u00e1 jsem tuto v\u00fdm\u011bnu nevystihl \u00fapln\u011b p\u0159esn\u011b. Nicm\u00e9n\u011b i kdy\u017e je debata-kter\u00e1-nebyla zaj\u00edmav\u00fdm p\u0159\u00edb\u011bhem, nen\u00ed m\u00fdm hlavn\u00edm c\u00edlem. Ve skute\u010dnosti bych celou tuto situaci se Stevem Kirschem nechal v odpadkov\u00e9m ko\u0161i, kam pat\u0159\u00ed, dokud bych nevid\u011bl jeho rozlu\u010dkov\u00e9 v\u00fdst\u0159ely na adresu komunity “virus neexistuje”. Ve snaze zachovat si tv\u00e1\u0159 t\u00edm, \u017ee p\u0159enese odpov\u011bdnost za debatu s Christine a jej\u00edmi odborn\u00edky na sv\u00e9 \u010dten\u00e1\u0159e (co\u017e by nem\u011blo b\u00fdt \u0161okuj\u00edc\u00ed, proto\u017ee v p\u0159en\u00e1\u0161en\u00ed odpov\u011bdnosti na “odborn\u00edky” je zjevn\u011b zku\u0161en\u00fd), se Steve pod\u011blil o dal\u0161\u00ed obskurn\u00ed tvrzen\u00ed sv\u00fdch “odborn\u00edk\u016f” ohledn\u011b \u010di\u0161t\u011bn\u00ed “vir\u016f”. Zde je n\u011bkolik stru\u010dn\u00fdch informac\u00ed z jeho p\u0159\u00edsp\u011bvku:<\/p>\n\n\n\nChce n\u011bkdo diskutovat na t\u00e9ma “Existuje virus?”.<\/strong><\/p>\n\n\n\nPokud samoz\u0159ejm\u011b existuje, ale existuj\u00ed stoupenci Sam Bailey, Stefana Lanky, Thomase Cowana, Andrewa Kaufmana a Christine Masseyov\u00e9, kte\u0159\u00ed tvrd\u00ed, \u017ee ne.<\/em><\/strong><\/p>\n\n\n\n“Nejsem ochoten investovat sv\u016fj \u010das do t\u00e9to debaty<\/em><\/strong>, ale pokud chcete vyzvat Sam Bailey, Stefana Lanku, Thomase Cowana, Andrewa Kaufmana a Christine Masseyovou, dejte mi pros\u00edm v\u011bd\u011bt v koment\u00e1\u0159\u00edch.”<\/p>\n\n\n\n“V podstat\u011b je \u010di\u0161t\u011bn\u00ed viru obt\u00ed\u017en\u00e9 a v dne\u0161n\u00edm sv\u011bt\u011b k n\u011bmu nen\u00ed d\u016fvod, tak\u017ee se ned\u011bl\u00e1. <\/em><\/strong>\u017d\u00e1dosti FOIA, kter\u00e9 vyd\u00e1vaj\u00ed, jsou reklamn\u00edm trikem, o n\u011bm\u017e v\u011bd\u00ed, \u017ee sel\u017ee. Je to od nich velmi neup\u0159\u00edmn\u00e9, \u017ee to nezve\u0159ej\u0148uj\u00ed.”<\/p>\n\n\n\n“Tak\u00e9 lid\u00e9, se kter\u00fdmi mluv\u00edm, pln\u011b uzn\u00e1vaj\u00ed, \u017ee \u017e\u00e1dn\u00fd vy\u010di\u0161t\u011bn\u00fd virus neexistuje<\/em><\/strong>, ale \u017ee nen\u00ed pot\u0159eba, proto\u017ee v\u0161echno, co pot\u0159ebuj\u00ed, mohou d\u011blat i bez n\u011bj. Lanka a spol. tvrd\u00ed, \u017ee je pot\u0159eba. Tak\u017ee te\u010f u\u017e je to jen ot\u00e1zka n\u00e1zoru. Ani jedna strana druhou nep\u0159esv\u011bd\u010d\u00ed. To se stalo.”<\/p>\n\n\n\n“D\u016fvod, pro\u010d nikdo virus nep\u0159e\u010distil, je ten, \u017ee v dne\u0161n\u00edm sv\u011bt\u011b, kde je snadno dostupn\u00e9 sekvenov\u00e1n\u00ed gen\u016f, to nen\u00ed pot\u0159eba.”<\/em><\/strong><\/p>\n\n\n\n<a href=\"https:\/\/stevekirsch.substack.com\/p\/does-anyone-want-to-debate-does-the\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/stevekirsch.substack.com\/p\/does-anyone-want-to-debate-does-the<\/a><\/p>\n\n\n\nNejprve bych r\u00e1d upozornil na Stev\u016fv zjevn\u00fd freudovsk\u00fd p\u0159e\u0161lap p\u0159i pokusu prohl\u00e1sit, \u017ee “virus” existuje: “Pokud<\/strong> samoz\u0159ejm\u011b existuje.” Z m\u00e9 strany se nejedn\u00e1 o p\u0159eklep. Nejsem tu od toho, abych si hr\u00e1l na gramatickou policii, proto\u017ee s\u00e1m d\u011bl\u00e1m spoustu pravopisn\u00fdch chyb. Jen jsem si myslel, \u017ee je to z\u00e1bavn\u011b ironick\u00fd zp\u016fsob, jak za\u010d\u00edt sv\u016fj p\u0159\u00edsp\u011bvek. Kdy\u017e u\u017e Steve nen\u00ed ochoten investovat sv\u016fj \u010das do debaty, mo\u017en\u00e1 by ho mohl v\u011bnovat korektu\u0159e?<\/p>\n\n\n\nTe\u010f, kdy\u017e u\u017e je legrace za n\u00e1mi, p\u0159ejd\u011bme k podstat\u011b v\u011bci ohledn\u011b \u010di\u0161t\u011bn\u00ed “vir\u016f”. Podle Stevov\u00fdch “odborn\u00edk\u016f” je \u010di\u0161t\u011bn\u00ed “viru” p\u0159\u00edli\u0161 obt\u00ed\u017en\u00e9 a ji\u017e nen\u00ed nutn\u00e9. Domn\u00edvaj\u00ed se, \u017ee v dne\u0161n\u00edm sv\u011bt\u011b molekul\u00e1rn\u00ed virologie k purifikaci “vir\u016f” nemus\u00ed doch\u00e1zet, proto\u017ee genom lze z\u00edskat z genetick\u00e9 pol\u00e9vky pln\u00e9 hostitelsk\u00e9 a jin\u00e9 nezn\u00e1m\u00e9 “nevirov\u00e9” RNA\/DNA. Domn\u00edvaj\u00ed se, \u017ee je mo\u017en\u00e9 z\u00edskat genom nezn\u00e1m\u00e9ho “viru” jeho poskl\u00e1d\u00e1n\u00edm z milion\u016f \u010dten\u00ed n\u00e1hodn\u00e9 RNA z\u00edskan\u00e9 z t\u011bchto nesouvisej\u00edc\u00edch zdroj\u016f ve vzorku. Steve a spol. tak cht\u011bj\u00ed, abyste v\u011b\u0159ili, \u017ee purifikace, tj. samotn\u00e9 kroky pou\u017e\u00edvan\u00e9 k odstran\u011bn\u00ed kontaminant\u016f, zne\u010di\u0161\u0165uj\u00edc\u00edch l\u00e1tek, cizorod\u00e9ho materi\u00e1lu atd. za \u00fa\u010delem izolace<\/strong> vzorku, ji\u017e nejsou nutn\u00e9, proto\u017ee technologie pokro\u010dila nad r\u00e1mec t\u011bchto primitivn\u00edch metod. Ponech\u00e1me-li stranou skute\u010dnost, \u017ee p\u0159izn\u00e1n\u00ed Steva a spol., \u017ee purifikovan\u00fd “SARS-COV-2” neexistuje, ni\u010d\u00ed jejich p\u0159edchoz\u00ed tvrzen\u00ed o izolaci “viru”, obstoj\u00ed Stevovy “odborn\u00e9” rady ohledn\u011b purifikace?<\/p>\n\n\n\nNe. V\u016fbec ne. Alespo\u0148 ne podle t\u011bchto odborn\u00edk\u016f:<\/p>\n\n\n\n“To, \u017ee takov\u00e1 “purifikace” je nezbytn\u00fdm p\u0159edpokladem<\/strong> pro detekci vir\u016f a vytvo\u0159en\u00ed platn\u00fdch protil\u00e1tkov\u00fdch a PCR test\u016f na jejich z\u00e1klad\u011b, tvrd\u00ed i v\u011bdci, kte\u0159\u00ed jsou nejrenomovan\u011bj\u0161\u00ed na sv\u011bt\u011b, mj:<\/p>\n\n\n\nWhite a Fenner<\/strong>: “Je to nezbytn\u00fd p\u0159edpoklad.”<\/p>\n\n\n\n “Je to nezbytn\u00e9.”<\/p>\n\n\n\nRobert Gallo<\/strong>: “Je t\u0159eba purifikovat.”<\/p>\n\n\n\nMarcel Tanner<\/strong>: “Pokud se \u010dist\u00fd izol\u00e1t SARS-CoV-2 nepoda\u0159\u00ed zdokumentovat v IVI [=\u00dastav virologie a imunologie] v Bernu, m\u00e1me probl\u00e9m.” (siehe zde <a href=\"https:\/\/www.youtube.com\/watch?v=TQRbxZy-8FE\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.youtube.com\/watch?v=TQRbxZy-8FE<\/a>).<\/p>\n\n\n\nFran\u00e7oise Barr\u00e9-Sinoussiov\u00e1<\/strong>: “… je t\u0159eba virus ze v\u0161eho toho nepo\u0159\u00e1dku vy\u010distit.” (pozn. p\u0159ekl.)<\/p>\n\n\n\nJean-Claude Chermann<\/strong>: “Ano, samoz\u0159ejm\u011b… Rozhodn\u011b.”<\/p>\n\n\n\nDavid Gordon<\/strong>: “Je to p\u0159irozen\u00fd krok od z\u00edsk\u00e1n\u00ed viru v bun\u011b\u010dn\u00e9 kultu\u0159e k n\u00e1sledn\u00e9mu z\u00edsk\u00e1n\u00ed purifikovan\u00e9ho viru.”<\/p>\n\n\n\nDominic Dwyer<\/strong>: “Purifikace, jak jen to jde, je d\u016fle\u017eit\u00e1 p\u0159i anal\u00fdze jak\u00e9hokoli viru nebo bakterie, kdy\u017e na to p\u0159ijde, dob\u0159e.”<\/p>\n\n\n\nWan Beom Park<\/strong>: “V situaci vypuknut\u00ed epidemie je izolace p\u0159\u00ed\u010dinn\u00e9ho viru nezbytn\u00e1 pro v\u00fdvoj a hodnocen\u00ed diagnostick\u00fdch n\u00e1stroj\u016f, terapeutik a kandid\u00e1t\u016f na vakc\u00edny.”<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><a href=\"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-content\/uploads\/2022\/04\/pasted-image-0-162.png\"><img loading=\"lazy\" decoding=\"async\" width=\"720\" height=\"540\" src=\"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-content\/uploads\/2022\/04\/pasted-image-0-162.png\" alt=\"\" class=\"wp-image-1572\" srcset=\"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-content\/uploads\/2022\/04\/pasted-image-0-162.png 720w, http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-content\/uploads\/2022\/04\/pasted-image-0-162-300x225.png 300w\" sizes=\"auto, (max-width: 720px) 100vw, 720px\" \/><\/a><\/figure>\n\n\n\nNejsem si jist\u00fd, kdo jsou Stevovi “odborn\u00edci”, ale v\u00fd\u0161e uveden\u00ed lid\u00e9 jsou zn\u00e1m\u00ed a uzn\u00e1van\u00ed v\u011bdci a virologov\u00e9. I kdy\u017e mohou nesouhlasit s t\u00edm, \u017ee “viry” neexistuj\u00ed, v\u0161ichni uzn\u00e1vaj\u00ed, \u017ee purifikace “viru” je naprosto nezbytn\u00fdm a z\u00e1sadn\u00edm krokem. Je to nezbytn\u00fd p\u0159edpoklad.<\/p>\n\n\n\nV\u00fd\u0161e uveden\u00ed nejsou jedin\u00ed odborn\u00edci, kte\u0159\u00ed tvrd\u00ed, \u017ee purifikace je nezbytn\u00e1. Rozhovor s prof: Dr. Osamu Nakagomim z Nagasaki University Graduate School of Biomedical Sciences Molecular Epidemiology, kter\u00fd je odborn\u00edkem na danou problematiku, se uv\u00e1d\u00ed rovn\u011b\u017e toto:<\/p>\n\n\n\nZ\u00e1kladn\u00ed principy ultracentrifug\u00e1ln\u00ed purifikace vir\u016f.<\/strong><\/p>\n\n\n\n“V posledn\u00edch letech se ve v\u00fdzkumu vir\u016f stalo standardn\u00ed prax\u00ed purifikovat a analyzovat genomy a identifikovat viry ze vzork\u016f pomoc\u00ed komer\u010dn\u00edch souprav. Vzhledem k tomu, \u017ee u zaveden\u00fdch vir\u016f<\/strong> jsou ji\u017e zn\u00e1my jejich genomy, je identifikace vir\u016f mo\u017en\u00e1 i ve sm\u00ed\u0161en\u00e9m stavu. Pro podrobn\u00e9 zkoum\u00e1n\u00ed povahy vir\u016f je v\u0161ak nejprve nutn\u00e9 zu\u0161lechtit virov\u00e9 \u010d\u00e1stice, aby bylo mo\u017en\u00e9 z\u00edskat vysoce purifikovan\u00fd materi\u00e1l.”<\/strong><\/p>\n\n\n\nDiskutujte pros\u00edm o nezbytnosti ultracentrifugace p\u0159i v\u00fdzkumu vir\u016f.<\/strong><\/p>\n\n\n\n“P\u0159i extrakci genomu viru klasickou metodou je t\u0159eba \u010d\u00e1stice viru nejprve p\u0159e\u010distit<\/strong>. Pot\u00e9 se zkoum\u00e1 genom viru extrahovan\u00fd z \u010d\u00e1stic. Ultracentrifugace hraje v tomto procesu d\u016fle\u017eitou roli. Purifikace virov\u00fdch \u010d\u00e1stic umo\u017e\u0148uje od po\u010d\u00e1tku zajistit, \u017ee se jedn\u00e1 o genomy rotavir\u016f v \u010d\u00e1stic\u00edch viru.<\/strong> V sou\u010dasn\u00e9 dob\u011b se takov\u00e1 anal\u00fdza prov\u00e1d\u00ed t\u00e9m\u011b\u0159 v\u017edy po sp\u011b\u0161n\u00e9 extrakci genomu, ani\u017e by se vzorek skute\u010dn\u011b purifikoval.<\/strong> Tato praxe je b\u011b\u017en\u00e1, proto\u017ee genom rotaviru je dob\u0159e zn\u00e1m a je v\u0161eobecn\u011b zn\u00e1mo, \u017ee pokud je p\u0159\u00edtomen genom (obr. 1 ) charakteristick\u00fd pro rotavirus, nen\u00ed pochyb o tom, \u017ee je p\u0159\u00edtomen i v \u010d\u00e1stic\u00edch rotaviru. P\u0159edpokl\u00e1dejme v\u0161ak, \u017ee se nap\u0159\u00edklad zab\u00fdv\u00e1me probl\u00e9mem ur\u010den\u00ed, jak\u00e9 organely hostitelsk\u00e9 bu\u0148ky nebo proteiny a genomy viru jsou agregov\u00e1ny v infikovan\u00e9 bu\u0148ce, ultracentrifugace se st\u00e1v\u00e1 nezbytnou.<\/strong> Nav\u00edc p\u0159i studiu nov\u00fdch vir\u016f je st\u00e1le v\u00edce nutn\u00e9 zkoumat, zda je v \u010d\u00e1stici p\u0159\u00edtomen genom, \u010di nikoli. V takov\u00fdch p\u0159\u00edpadech se purifikace pomoc\u00ed ultracentrifugy st\u00e1v\u00e1 nezbytnost\u00ed. Informace o vztlakov\u00e9 hustot\u011b, velikosti a sedimenta\u010dn\u00edm koeficientu (Svedbergova hodnota, hodnota S), kter\u00e9 se v\u0161echny berou v \u00favahu p\u0159i ultracentrifugaci, jsou vlastn\u011b z\u00e1kladn\u00edm aspektem virologie, kter\u00e9 se dohromady naz\u00fdvaj\u00ed fyzik\u00e1ln\u011b-chemick\u00e9 vlastnosti vir\u016f.”<\/p>\n\n\n\n<a href=\"https:\/\/www.beckman.com\/resources\/reading-material\/interviews\/fundamentals-of-ultracentrifugal-virus-purification\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.beckman.com\/resources\/reading-material\/interviews\/fundamentals-of-ultracentrifugal-virus-purification<\/a><\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><a href=\"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-content\/uploads\/2022\/04\/pasted-image-0-163.png\"><img loading=\"lazy\" decoding=\"async\" width=\"520\" height=\"669\" src=\"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-content\/uploads\/2022\/04\/pasted-image-0-163.png\" alt=\"\" class=\"wp-image-1574\" srcset=\"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-content\/uploads\/2022\/04\/pasted-image-0-163.png 520w, http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-content\/uploads\/2022\/04\/pasted-image-0-163-233x300.png 233w\" sizes=\"auto, (max-width: 520px) 100vw, 520px\" \/><\/a><\/figure>\n\n\n\nZaj\u00edmalo by m\u011b, jestli by Steve a spol. dok\u00e1zali z t\u011bchto neo\u010di\u0161t\u011bn\u00fdch EM sn\u00edmk\u016f uk\u00e1zat na “SARS-COV-2”, kdybychom odstranili \u0161t\u00edtky?<\/strong><\/p>\n\n\n\nJak je vid\u011bt z vyj\u00e1d\u0159en\u00ed Dr. Osamu Nakagomiho i v\u00fd\u0161e uveden\u00fdch odborn\u00edk\u016f, purifikace je zcela nezbytn\u00e1, zejm\u00e9na v p\u0159\u00edpadech s “nov\u00fdmi viry”, jako je “SARS-COV-2”, kter\u00fd, jak Steve a spol. p\u0159izn\u00e1vaj\u00ed, nebyl nikdy purifikov\u00e1n<\/strong>. Bez purifikace je ve vzorku mnoho organel hostitelsk\u00e9 bu\u0148ky a dal\u0161\u00edch protein\u016f, mikroorganism\u016f, bakteri\u00ed atd., a proto nelze tvrdit, \u017ee byl izolov\u00e1n. Nebylo by mo\u017en\u00e9 ur\u010dit, \u017ee RNA pou\u017eit\u00e1 k vytvo\u0159en\u00ed genomu “SARS-COV-2” poch\u00e1z\u00ed z jednoho zdroje. Dr. Nakagomi vlastn\u011b uv\u00e1d\u00ed, \u017ee purifikace nen\u00ed nutn\u00e1 pouze tehdy, kdy\u017e je genom ji\u017e zn\u00e1m a stanoven, a purifikace je tedy nezbytn\u00fdm krokem k z\u00edsk\u00e1n\u00ed p\u016fvodn\u00edho genomu. P\u0159esto to vytv\u00e1\u0159\u00ed trochu h\u00e1danku. Kde bylo n\u011bkdy prok\u00e1z\u00e1no, \u017ee \u010d\u00e1stice pova\u017eovan\u00e9 za “viry” byly n\u011bkdy purifikov\u00e1ny a izolov\u00e1ny p\u0159\u00edmo z nemocn\u00e9ho \u010dlov\u011bka za \u00fa\u010delem z\u00edsk\u00e1n\u00ed p\u016fvodn\u00edho genomu jak\u00e9hokoli “viru”? V ur\u010dit\u00e9m okam\u017eiku v historii “virov\u00fdch” genom\u016f musel b\u00fdt tento proces \u010di\u0161t\u011bn\u00ed a izolace proveden p\u0159edt\u00edm, ne\u017e mohl b\u00fdt z\u00edsk\u00e1n jak\u00fdkoli genom jak\u00e9hokoli “viru” a pova\u017eov\u00e1n za p\u0159esn\u00fd a spolehliv\u00fd. <a href=\"https:\/\/viroliegy.com\/2022\/01\/22\/how-accurate-and-reliable-are-genomes\/\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/viroliegy.com\/2022\/01\/22\/how-accurate-and-reliable-are-genomes\/<\/a><\/p>\n\n\n\nNikdy v\u0161ak nebyl proveden, zejm\u00e9na u “koronavir\u016f”, jak jsem nast\u00ednil zde.<\/p>\n\n\n\n<figure class=\"wp-block-embed is-type-link is-provider-viroliegy wp-block-embed-viroliegy\"><div class=\"wp-block-embed__wrapper\">\n<a href=\"https:\/\/viroliegy.com\/2021\/12\/24\/creating-the-coronavirus\/\">Creating the “Coronavirus”<\/a>\n<\/div><\/figure>\n\n\n\nGenom “SARS-COV-2” neexistoval a jeho sekvence nebyla p\u0159edem zn\u00e1ma. Genom byl vytvo\u0159en z ne\u010di\u0161t\u011bn\u00e9 bronkoalveol\u00e1rn\u00ed tekutiny (BALF) od jednoho pacienta a poskl\u00e1d\u00e1n v po\u010d\u00edta\u010di z jin\u00fdch ne\u010di\u0161t\u011bn\u00fdch referen\u010dn\u00edch genom\u016f vytvo\u0159en\u00fdch podobn\u00fdm zp\u016fsobem. <a href=\"https:\/\/viroliegy.com\/2021\/09\/06\/creating-the-sars-cov-2-genome\/\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/viroliegy.com\/2021\/09\/06\/creating-the-sars-cov-2-genome\/<\/a><\/p>\n\n\n\nV dokumentu WHO t\u00fdkaj\u00edc\u00edm se sekvenov\u00e1n\u00ed genom\u016f pomoc\u00ed metagenomiky, jako tomu bylo u p\u016fvodn\u00edho genomu “SARS-COV-2”, se p\u0159ipou\u0161t\u00ed, \u017ee bude sekvenov\u00e1n i materi\u00e1l s vysok\u00fdm obsahem “nevirov\u00fdch” hostitel\u016f. Tvrd\u00ed, \u017ee se maj\u00ed prov\u00e1d\u011bt purifika\u010dn\u00ed kroky, jako je centrifugace a filtrace, ale i purifikace vzork\u016f povede k vysok\u00e9mu po\u010dtu “nec\u00edlov\u00fdch, nevirov\u00fdch” \u010dten\u00ed:<\/p>\n\n\n\nGenomick\u00e9 sekvenov\u00e1n\u00ed SARS-CoV-2<\/strong><\/p>\n\n\n\n“Odstran\u011bn\u00ed hostitele nebo jin\u00e9ho genetick\u00e9ho materi\u00e1lu, kter\u00fd nen\u00ed SARS-CoV-2, ve vzorku vede k vy\u0161\u0161\u00edmu pod\u00edlu \u010dten\u00ed SARS-CoV-2 v generovan\u00fdch sekvena\u010dn\u00edch datech, a tedy k vy\u0161\u0161\u00ed \u0161anci na z\u00edsk\u00e1n\u00ed \u00fapln\u00e9ho genomu. Metagenomick\u00e9 p\u0159\u00edstupy SARS-CoV-2 proto obvykle zahrnuj\u00ed kroky k odstran\u011bn\u00ed hostitelsk\u00fdch a bakteri\u00e1ln\u00edch bun\u011bk, a to bu\u010f odst\u0159ed\u011bn\u00edm nebo filtrac\u00ed p\u0159ed extrakc\u00ed RNA, nebo chemick\u00fdm \u010di enzymatick\u00fdm odstran\u011bn\u00edm ne\u017e\u00e1douc\u00ed DNA\/RNA. <\/strong>To je snaz\u0161\u00ed u tekut\u00fdch vzork\u016f, z nich\u017e lze bu\u0148ky snadn\u011bji odd\u011blit, jako je bronchoalveol\u00e1rn\u00ed lav\u00e1\u017e (tabulka 4). Obsah ribosom\u00e1ln\u00ed RNA (rRNA) a DNA se tak\u00e9 b\u011b\u017en\u011b ochuzuje p\u0159i p\u0159\u00edprav\u011b knihovny pro sekvenov\u00e1n\u00ed virov\u00e9 RNA a nosn\u00e1 RNA se z extrakc\u00ed \u010dasto vynech\u00e1v\u00e1 nebo se nahrazuje line\u00e1rn\u00edm polyakrylamidem. Navzdory t\u011bmto opat\u0159en\u00edm mohou vzorky st\u00e1le obsahovat velk\u00e9 mno\u017estv\u00ed nec\u00edlov\u00e9 hostitelsk\u00e9 DNA\/RNA, kter\u00e1 m\u016f\u017ee b\u00fdt rovn\u011b\u017e sekvenov\u00e1na.<\/strong> Metagenomick\u00e9 p\u0159\u00edstupy proto obecn\u011b t\u011b\u017e\u00ed ze vstupu vzork\u016f s vysokou virovou z\u00e1t\u011b\u017e\u00ed (tak, \u017ee p\u0159im\u011b\u0159en\u00fd pod\u00edl genetick\u00e9ho materi\u00e1lu ve vzorku tvo\u0159\u00ed virus).”<\/p>\n\n\n\n“Metagenomick\u00e9 sekvenov\u00e1n\u00ed obvykle produkuje vysok\u00e9 mno\u017estv\u00ed nec\u00edlov\u00fdch, nevirov\u00fdch \u010dten\u00ed. <\/strong>Je tak\u00e9 \u010dasto (i kdy\u017e ne v\u017edy, v z\u00e1vislosti na sekvena\u010dn\u00ed platform\u011b a multiplexov\u00e1n\u00ed) n\u00e1kladn\u011bj\u0161\u00ed ne\u017e p\u0159\u00edstupy zalo\u017een\u00e9 na c\u00edlen\u00e9m z\u00e1chytu nebo na amplikonov\u00e9m sekvenov\u00e1n\u00ed, proto\u017ee k vytvo\u0159en\u00ed jednoho genomu SARS-CoV-2 je t\u0159eba z\u00edskat v\u00edce dat. Nav\u00edc kroky p\u0159ed\u00fapravy, kter\u00e9 jsou zvl\u00e1\u0161t\u011b v\u00fdhodn\u00e9 pro metagenomiku, jako je centrifugace, se obvykle neprov\u00e1d\u011bj\u00ed pro molekul\u00e1rn\u00ed diagnostick\u00e9 testy, tak\u017ee pro metagenomick\u00e9 sekvenov\u00e1n\u00ed m\u016f\u017ee b\u00fdt nutn\u00e9 prov\u00e1d\u011bt nov\u00e9 extrakce, kter\u00e9 zahrnuj\u00ed kroky p\u0159ed\u00fapravy.”<\/strong><\/p>\n\n\n\n<a href=\"https:\/\/www.who.int\/publications-detail-redirect\/9789240018440\">https:\/\/www.google.com\/url?sa=t&source=web&rct=j&url=https:\/\/www.who.int\/publications-detail-redirect\/9789240018440&ved=2ahUKEwjB9N-JgOjyAhVWvZ4KHVWZBckQFnoECB4QAQ&usg=AOvVaw28OrakzQD6BVezIuZC15Jw<\/a><\/p>\n\n\n\nJin\u00fd zdroj o v\u00fdhod\u00e1ch a nev\u00fdhod\u00e1ch genomick\u00e9ho sekvenov\u00e1n\u00ed uv\u00e1d\u00ed, \u017ee kontaminace, nap\u0159\u00edklad bakteriemi, kter\u00e9 jsou bez purifikace jist\u011b p\u0159\u00edtomny, povede k nep\u0159esn\u00fdm genom\u016fm:<\/p>\n\n\n\nV\u00fdhody a omezen\u00ed sekvenov\u00e1n\u00ed genom\u016f<\/strong><\/p>\n\n\n\n“Faktory mimo kontrolu poskytovatele slu\u017eeb pov\u011b\u0159en\u00e9ho izolac\u00ed a sekvenov\u00e1n\u00edm DNA mohou negativn\u011b ovlivnit kvalitu sekvence genomu, a tedy i jej\u00ed interpretaci.<\/strong> M\u016f\u017ee j\u00edt nap\u0159\u00edklad o kvalitu vzorku DNA poskytnut\u00e9ho k anal\u00fdze, jako je n\u00edzk\u00e9 mno\u017estv\u00ed, vysok\u00e1 bakteri\u00e1ln\u00ed kontaminace<\/strong> nebo degradace vzorku. Takov\u00e9 faktory mohou dokonce zabr\u00e1nit proveden\u00ed postupu.<\/strong> Za takov\u00fdch okolnost\u00ed m\u016f\u017ee b\u00fdt klient nucen dodat nov\u00fd vzorek.”<\/p>\n\n\n\n<a href=\"https:\/\/merogenomics.ca\/en\/advantages-and-limitations-of-genome-sequencing\/\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/merogenomics.ca\/en\/advantages-and-limitations-of-genome-sequencing\/<\/a><\/p>\n\n\n\nVzhledem k tomu, \u017ee Steve a spol. p\u0159izn\u00e1vaj\u00ed, \u017ee “SARS-COV-2” nebyl nikdy purifikov\u00e1n, av\u0161ak purifikace je u “nov\u00fdch vir\u016f” nezbytnou podm\u00ednkou pro z\u00edsk\u00e1n\u00ed p\u0159esn\u00e9ho genomu, jak mohou tvrdit, \u017ee tento krok nen\u00ed nutn\u00fd?<\/p>\n\n\n\nPravd\u011bpodobn\u011b je to zp\u016fsobeno jinou skute\u010dnost\u00ed, kterou Steve p\u0159iznal: purifikace je obt\u00ed\u017en\u00e1.<\/strong> J\u00e1 bych v\u0161ak \u0161el je\u0161t\u011b o krok d\u00e1l a \u0159ekl bych, \u017ee p\u0159i pr\u00e1ci s \u010d\u00e1sticemi o velikosti nano je purifikace nemo\u017en\u00e1. <\/strong>V tomto p\u0159\u00edsp\u011bvku nebudu zach\u00e1zet do p\u0159\u00edli\u0161n\u00fdch podrobnost\u00ed, proto\u017ee probl\u00e9my s \u010di\u0161t\u011bn\u00edm jsem nast\u00ednil zde <a href=\"https:\/\/viroliegy.com\/2021\/09\/20\/is-complete-purification-isolation-of-a-virus-even-possible\/\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/viroliegy.com\/2021\/09\/20\/is-complete-purification-isolation-of-a-virus-even-possible\/<\/a><\/p>\n\n\n\na zde <a href=\"https:\/\/viroliegy.com\/2021\/09\/21\/the-purification-problem\/\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/viroliegy.com\/2021\/09\/21\/the-purification-problem\/<\/a>. Bylo v\u0161ak mnohokr\u00e1t p\u0159izn\u00e1no, \u017ee je nemo\u017en\u00e9 odd\u011blit “viry” od exozom\u016f <a href=\"https:\/\/viroliegy.com\/2021\/09\/10\/viruses-or-exosomes\/\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/viroliegy.com\/2021\/09\/10\/viruses-or-exosomes\/<\/a><\/p>\n\n\n\na dal\u0161\u00edch extracelul\u00e1rn\u00edch vezikul, kter\u00e9 spolu sedimentuj\u00ed. Neexistuje \u017e\u00e1dn\u00e1 metoda, a\u0165 u\u017e ultracentrifugace, filtrace, sr\u00e1\u017een\u00ed atd., kter\u00e1 by dok\u00e1zala “viry” zcela vy\u010distit. A\u010dkoli podobn\u00e1 tvrzen\u00ed najdete v n\u011bkter\u00fdch p\u0159\u00edsp\u011bvc\u00edch, na kter\u00e9 jsem odkazoval, uvedu ned\u00e1vn\u00fd \u010dl\u00e1nek, kter\u00fd se zam\u011b\u0159il na pot\u0159ebu purifikace RNA pro epigenetick\u00e9 studie. Auto\u0159i uv\u00e1d\u011bj\u00ed r\u016fzn\u00e9 purifika\u010dn\u00ed metody a pak p\u0159izn\u00e1vaj\u00ed, \u017ee \u017e\u00e1dn\u00e1 z nich sama o sob\u011b nen\u00ed dostate\u010dn\u00e1 k o\u010di\u0161t\u011bn\u00ed “vir\u016f” od ne\u010distot poch\u00e1zej\u00edc\u00edch z hostitele. Tyto ne\u010distoty pak ovliv\u0148uj\u00ed tvorbu genomu a jakoukoli studii s n\u00edm souvisej\u00edc\u00ed. Dokonce i v p\u0159\u00edpad\u011b kombinace mohou pouze tvrdit, \u017ee tyto metody zv\u00fd\u0161\u00ed v\u00fdt\u011b\u017enost a kvalitu “vir\u016f”, nikoliv \u017ee \u010d\u00e1stice zcela vy\u010dist\u00ed.<\/p>\n\n\n\n“Relativn\u011b n\u00edzk\u00fd v\u00fdskyt virov\u00e9ho genomov\u00e9ho materi\u00e1lu v prost\u0159ed\u00ed nukleov\u00fdch kyselin klinick\u00fdch vzork\u016f klade omezen\u00ed na vyu\u017eitelnost aplikac\u00ed souvisej\u00edc\u00edch s epigenetikou, jako je ELISA na metylaci m6A RNA <a href=\"https:\/\/www.epigentek.com\/catalog\/epiquik-m6a-rna-methylation-quantification-kit-colorimetric-p-3646.html\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.epigentek.com\/catalog\/epiquik-m6a-rna-methylation-quantification-kit-colorimetric-p-3646.html<\/a>, pro specifick\u00e9 studium virov\u00e9ho epigenomu. Takov\u00e9 testy vy\u017eaduj\u00ed vysoce \u010dist\u00fd vstupn\u00ed materi\u00e1l bez ne\u010distot poch\u00e1zej\u00edc\u00edch od hostitele, jejich\u017e epigenetick\u00e9 modifikace mohou b\u00fdt rovn\u011b\u017e detekov\u00e1ny a ru\u0161it v\u00fdsledky.”<\/strong><\/p>\n\n\n\n“V\u00fd\u0161e uveden\u00e9 metody obecn\u011b nesta\u010d\u00ed, jsou-li prov\u00e1d\u011bny samostatn\u011b, k adekv\u00e1tn\u00edmu p\u0159e\u010di\u0161t\u011bn\u00ed vir\u016f.<\/strong> Studie zam\u011b\u0159en\u00e9 na epigenom viru vy\u017eaduj\u00ed vysoce \u010dist\u00fd vstupn\u00ed materi\u00e1l bez interferenc\u00ed s epigenetick\u00fdmi modifikacemi hostitelsk\u00e9 DNA, RNA nebo proteinu. <\/strong>Kombinace v\u00fd\u0161e uveden\u00fdch metod m\u016f\u017ee zv\u00fd\u0161it v\u00fdt\u011b\u017enost, v\u00fdt\u011b\u017enost a kvalitu vir\u016f.”<\/p>\n\n\n\n<a href=\"https:\/\/www.epigentek.com\/catalog\/methods-of-virus-Purification-n-41.html\">https:\/\/www.epigentek.com\/catalog\/methods-of-virus-Purification-n-41.html<\/a><\/p>\n\n\n\nI kdy\u017e se na vzorc\u00edch provedou purifika\u010dn\u00ed kroky, v\u017edy bude ve vzorku mnoho zn\u00e1m\u00fdch i nezn\u00e1m\u00fdch identick\u00fdch \u010d\u00e1stic s r\u016fzn\u00fdmi zdroji genetick\u00e9ho materi\u00e1lu. Kontaminace je roz\u0161\u00ed\u0159en\u00fdm probl\u00e9mem jak p\u0159i kultivaci bun\u011bk, tak v genomice <a href=\"https:\/\/viroliegy.com\/2022\/01\/24\/genome-contamination-a-widespread-problem\/\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/viroliegy.com\/2022\/01\/24\/genome-contamination-a-widespread-problem\/<\/a>. D\u00edky tomu je zobrazov\u00e1n\u00ed elektronovou mikroskopi\u00ed a vytv\u00e1\u0159en\u00ed genomu naprosto nesmysln\u00e9 a nepou\u017eiteln\u00e9 jako d\u016fkaz “viru”. Abychom tento bod zd\u016fraznili, uv\u00e1d\u00edme n\u011bkolik nejd\u016fle\u017eit\u011bj\u0161\u00edch informac\u00ed z Manuela z roku 1996 o purifikaci “vir\u016f”:<\/p>\n\n\n\n“Purifikace viru je fyzick\u00e1 separace viru v koncentrovan\u00e9 form\u011b od prost\u0159ed\u00ed hostitelsk\u00e9 bu\u0148ky, ve kter\u00e9m vyrostl. Viry je t\u0159eba purifikovat pro mnoho studi\u00ed, p\u0159i nich\u017e je t\u0159eba odli\u0161it vlastnosti nebo strukturu viru od vlastnost\u00ed nebo struktury hostitelsk\u00fdch bun\u011bk nebo kultiva\u010dn\u00edho prost\u0159ed\u00ed, jako jsou anal\u00fdzy struktury virov\u00fdch polypeptid\u016f, funkce membr\u00e1ny glykoprotein\u016f atd.”<\/strong><\/p>\n\n\n\nKrit\u00e9ria \u010distoty<\/strong><\/p>\n\n\n\n“Pozorov\u00e1n\u00ed \u010d\u00e1stic v elektronov\u00e9m mikroskopu sice nen\u00ed dobr\u00fdm krit\u00e9riem \u010distoty, ale umo\u017e\u0148uje odhalit “ne\u017e\u00e1douc\u00ed struktury”.<\/strong><\/p>\n\n\n\nDalo by se o\u010dek\u00e1vat, \u017ee slo\u017eky m\u00e9dia budou tvo\u0159it hlavn\u00ed \u010d\u00e1st kontaminant\u016f purifikovan\u00fdch virov\u00fdch prepar\u00e1t\u016f. <\/strong>To lze sledovat pomoc\u00ed gelov\u00fdch difuzn\u00edch test\u016f, kdy lze s virov\u00fdm prepar\u00e1tem reagovat antis\u00e9rami nam\u00ed\u0159en\u00fdmi nap\u0159. proti telec\u00edmu s\u00e9ru nebo neinfikovan\u00fdm bu\u0148k\u00e1m.”<\/p>\n\n\n\n<a href=\"https:\/\/dx.doi.org\/10.1016%2FB978-012465330-6%2F50005-1\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/dx.doi.org\/10.1016%2FB978-012465330-6%2F50005-1<\/a><\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><a href=\"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-content\/uploads\/2022\/04\/pasted-image-0-164.png\"><img loading=\"lazy\" decoding=\"async\" width=\"654\" height=\"781\" src=\"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-content\/uploads\/2022\/04\/pasted-image-0-164.png\" alt=\"\" class=\"wp-image-1575\" srcset=\"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-content\/uploads\/2022\/04\/pasted-image-0-164.png 654w, http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-content\/uploads\/2022\/04\/pasted-image-0-164-251x300.png 251w\" sizes=\"auto, (max-width: 654px) 100vw, 654px\" \/><\/a><\/figure>\n\n\n\nJak je vid\u011bt, “viry” mus\u00ed b\u00fdt purifikov\u00e1ny, aby bylo mo\u017en\u00e9 odli\u0161it strukturu a fyzik\u00e1ln\u00ed vlastnosti “viru” od hostitelsk\u00fdch bun\u011bk a kultiva\u010dn\u00edho m\u00e9dia. \u0158\u00edk\u00e1 se, \u017ee slo\u017eky kultiva\u010dn\u00edho m\u00e9dia tvo\u0159\u00ed v\u011bt\u0161inu kontaminant\u016f v purifikovan\u00e9m “viru”. Pat\u0159\u00ed sem fet\u00e1ln\u00ed hov\u011bz\u00ed s\u00e9rum, kter\u00e9 se p\u0159id\u00e1v\u00e1 t\u00e9m\u011b\u0159 do ka\u017ed\u00e9 kultury a kter\u00e9 je zcela odd\u011blen\u00fdm zdrojem RNA od hostitelsk\u00e9ho zdroje. Nezmi\u0148uj\u00ed se o p\u0159idan\u00e9 \u017eivo\u010di\u0161n\u00e9 RNA, kter\u00e1 by poch\u00e1zela z bun\u011bk Vero pravideln\u011b pou\u017e\u00edvan\u00fdch ke kultivaci, jako v p\u0159\u00edpad\u011b “SARS-COV-2”. Ve\u0161ker\u00fd tento “nevirov\u00fd” materi\u00e1l by musel b\u00fdt nejprve vylou\u010den spolu s materi\u00e1lem hostitele, stejn\u011b jako mo\u017en\u00e1 kontaminace bakteriemi, exozomy, MVB, jin\u00fdmi mikroorganismy atd., ne\u017e by mohl b\u00fdt genom pova\u017eov\u00e1n za platn\u00fd. Jinak neexistuje re\u00e1ln\u00fd zp\u016fsob, jak zjistit, kter\u00e1 RNA pat\u0159\u00ed ke kter\u00e9mu zdroji ve sm\u011bsi a zda po\u010d\u00edta\u010dem vytvo\u0159en\u00fd genom nen\u00ed spojen\u00edm RNA se\u0161it\u00fdch z t\u011bchto \u010detn\u00fdch zdroj\u016f.<\/p>\n\n\n\nJe z\u0159ejm\u00e9, \u017ee purifikace je naprosto nezbytn\u00fd proces, i kdy\u017e samotn\u00e9 metody jsou chybn\u00e9 a nedok\u00e1\u017eou tyto prepar\u00e1ty zcela vy\u010distit. Proto Steve a spol. tvrd\u00ed, \u017ee je “obt\u00ed\u017en\u00e9” (tj. nemo\u017en\u00e9) purifikovat “viry”, \u017ee to ji\u017e nen\u00ed nutn\u00e9 a pro\u010d cht\u011bj\u00ed tento krok zcela p\u0159esko\u010dit. V\u011bd\u00ed, \u017ee je to nemo\u017en\u00e9. V\u011bd\u00ed, \u017ee nemohou p\u0159edlo\u017eit jedinou studii, kde by \u010d\u00e1stice, o nich\u017e tvrd\u00ed, \u017ee jsou “viry”, byly zcela p\u0159e\u010di\u0161t\u011bny a izolov\u00e1ny p\u0159\u00edmo z nemocn\u00e9ho hostitele. Nemohou to prok\u00e1zat ani v prac\u00edch, kde jsou “viry” kultivov\u00e1ny. Cht\u011bj\u00ed, abyste uv\u011b\u0159ili, \u017ee technologie pokro\u010dila natolik, \u017ee dok\u00e1\u017ee tyto nep\u0159e\u010di\u0161t\u011bn\u00e9 sm\u011bsi RNA vyb\u00edrat, aby z nich poskl\u00e1dala teoretickou p\u0159edstavu neviditeln\u00e9ho “viru” v podob\u011b genomu. I kdyby to byl logick\u00fd argument (nen\u00ed), genom z nep\u0159e\u010di\u0161t\u011bn\u00fdch vzork\u016f by byl p\u0159inejlep\u0161\u00edm NEP\u0158\u00cdM\u00ddM d\u016fkazem, nikoliv P\u0158\u00cdM\u00ddM d\u016fkazem “viru”.<\/p>\n\n\n\nNa\u0161t\u011bst\u00ed i bez ohledu na zdroje, o kter\u00e9 jsem se pod\u011blil v\u00fd\u0161e a kter\u00e9 Steva a spol. zcela zpochyb\u0148uj\u00ed, se m\u016f\u017eeme spolehnout na logiku a kritick\u00e9 my\u0161len\u00ed, abychom pochopili, \u017ee jejich tvrzen\u00ed jsou sm\u011b\u0161n\u00e1.  Aby mohl b\u00fdt genom pova\u017eov\u00e1n za platn\u00fd d\u016fkaz, mus\u00ed b\u00fdt nejprve prok\u00e1z\u00e1no, \u017ee sekvenovan\u00e1 entita skute\u010dn\u011b existuje ve skute\u010dnosti. Nelze prost\u011b od za\u010d\u00e1tku p\u0159edpokl\u00e1dat, \u017ee se v neo\u010di\u0161t\u011bn\u00e9m vzorku nach\u00e1z\u00ed neviditeln\u00fd “virus”, ani\u017e by se v\u016fbec ov\u011b\u0159ilo, \u017ee skute\u010dn\u011b existuje. To vy\u017eaduje, aby \u010d\u00e1stice, o nich\u017e se tvrd\u00ed, \u017ee jsou “viry”, byly nalezeny ve stavu zcela prost\u00e9m kontaminant\u016f, zne\u010di\u0161\u0165uj\u00edc\u00edch l\u00e1tek a cizorod\u00e9ho materi\u00e1lu a tak\u00e9 odd\u011bleny od v\u0161eho ostatn\u00edho. Aby k tomu mohlo doj\u00edt, mus\u00ed vzorek proj\u00edt kroky purifikace (odst\u0159ed\u011bn\u00ed, filtrace, vysr\u00e1\u017een\u00ed atd.), aby bylo mo\u017en\u00e9 prok\u00e1zat, \u017ee existuje v izolovan\u00e9m stavu. Teprve pot\u00e9 lze z\u00edskat d\u016fkaz patogenity pomoc\u00ed purifikovan\u00fdch \u010d\u00e1stic jako platn\u00e9 nez\u00e1visl\u00e9 prom\u011bnn\u00e9, aby bylo mo\u017en\u00e9 stanovit p\u0159\u00ed\u010dinu a n\u00e1sledek. Teprve potom lze \u0159\u00edci, \u017ee \u010d\u00e1stice identifikovan\u00e9 na EM sn\u00edmc\u00edch jsou “virus”. Teprve pak by bylo mo\u017en\u00e9 z\u00edskat genom. Teprve pak lze “virus” pln\u011b charakterizovat.<\/p>\n\n\n\nBez purifikace Steve a spol. \u017e\u00e1dn\u00fd “virus” nemaj\u00ed.<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><a href=\"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-content\/uploads\/2022\/04\/pasted-image-0-165.png\"><img loading=\"lazy\" decoding=\"async\" width=\"850\" height=\"400\" src=\"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-content\/uploads\/2022\/04\/pasted-image-0-165.png\" alt=\"\" class=\"wp-image-1576\" srcset=\"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-content\/uploads\/2022\/04\/pasted-image-0-165.png 850w, http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-content\/uploads\/2022\/04\/pasted-image-0-165-300x141.png 300w, http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-content\/uploads\/2022\/04\/pasted-image-0-165-768x361.png 768w\" sizes=\"auto, (max-width: 850px) 100vw, 850px\" \/><\/a><\/figure>\n\n\n\nA tak se dost\u00e1v\u00e1me k j\u00e1dru probl\u00e9mu, kdy se spol\u00e9h\u00e1me na to, \u017ee “odborn\u00edci” budou p\u0159em\u00fd\u0161let za n\u00e1s. Steve se spol\u00e9hal na sv\u00e9 “odborn\u00edky”, kte\u0159\u00ed mu \u0159ekli, \u017ee proces purifikace nen\u00ed nutn\u00fd. Dovolil “odborn\u00edk\u016fm”, aby mu \u0159ekli, \u017ee definice izolace znamen\u00e1 s\u010d\u00edt\u00e1n\u00ed mnoha v\u011bc\u00ed dohromady a ne to, co ve skute\u010dnosti znamen\u00e1, co\u017e je existence ve stavu odd\u011blen\u00e9m od v\u0161eho ostatn\u00edho. Neud\u011blal ani zb\u011b\u017en\u00fd pr\u016fzkum, aby pochopil, \u017ee jeho takzvan\u00ed “odborn\u00edci” se m\u00fdl\u00ed. Jejich nep\u0159esn\u00e1 tvrzen\u00ed v\u0161ak nyn\u00ed mus\u00ed obhajovat on. Steve je bohu\u017eel neoblomn\u00fd v tom, \u017ee zat\u00edmco byl ochoten investovat \u010das do naps\u00e1n\u00ed blogov\u00e9ho p\u0159\u00edsp\u011bvku o sv\u00e9 neochot\u011b k diskusi, nen\u00ed ochoten investovat sv\u016fj \u010das do skute\u010dn\u00e9 obhajoby sv\u00fdch tvrzen\u00ed v diskusi. Vid\u00edm to tedy tak, \u017ee Steve m\u00e1 t\u0159i mo\u017enosti:<\/p>\n\n\n\nNaj\u00edt si \u010das na debatu s Christine a jej\u00edmi odborn\u00edky, aby obh\u00e1jil sv\u00e1 sm\u011b\u0161n\u00e1 tvrzen\u00ed.<\/p>\n\n\n\nNaj\u00edt nov\u00e9 “odborn\u00edky”, kte\u0159\u00ed rozum\u00ed metod\u00e1m pou\u017e\u00edvan\u00fdm pro \u010di\u0161t\u011bn\u00ed a izolaci “vir\u016f” a d\u016fvod\u016fm, pro\u010d jsou nezbytn\u00e9.<\/p>\n\n\n\nNaj\u00edt si \u010das na vlastn\u00ed v\u00fdzkum a pou\u017e\u00edt kritick\u00e9 my\u0161len\u00ed a logiku, aby s\u00e1m rozpoznal pravdu, m\u00edsto aby se spol\u00e9hal na “odborn\u00edky”, kte\u0159\u00ed to ud\u011blaj\u00ed za n\u011bj.<\/p>\n\n\n\nDouf\u00e1m, \u017ee Steve zvol\u00ed mo\u017enost \u010d. 3. Nicm\u00e9n\u011b moc nad\u011bj\u00ed tomu ned\u00e1v\u00e1m.<\/p>\n","protected":false},"excerpt":{"rendered":"Po m\u00e9 reakci na jeho tvrzen\u00ed, \u017ee “SARS-COV-2” byl izolov\u00e1n, jsem nem\u011bl v pl\u00e1nu ps\u00e1t dal\u0161\u00ed \u010dl\u00e1nky ani v\u011bnovat Stevu Kirschovi v\u00edce sv\u00e9ho \u010dasu. Po p\u0159e\u010dten\u00ed jeho p\u0159\u00edsp\u011bvku na blogu mi bylo jasn\u00e9, \u017ee nerozum\u00ed tomu, o \u010dem p\u00ed\u0161e. I kdyby to nebylo jasn\u00e9 nikomu, kdo to \u010dte, Steve si dovolil otev\u0159en\u011b p\u0159iznat, \u017ee t\u00e9matu nerozum\u00ed, proto\u017ee se spol\u00e9h\u00e1 na “odborn\u00edky”, kte\u0159\u00ed mu \u0159\u00edkaj\u00ed, co si m\u00e1 myslet a \u010demu m\u00e1 v\u011b\u0159it:<\/p>\n","protected":false},"author":4,"featured_media":1571,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_expiration-date-status":"","_expiration-date":1651133487,"_expiration-date-type":"draft","_expiration-date-categories":[],"_expiration-date-options":[]},"categories":[462],"tags":[718,467,644,466,498,717,432,474],"class_list":["post-1570","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-medicina","tag-christina-massey","tag-kovid","tag-mike-stone","tag-podvod","tag-sars-cov-2","tag-steve-kirsch","tag-veda","tag-virologie"],"_links":{"self":[{"href":"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-json\/wp\/v2\/posts\/1570","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-json\/wp\/v2\/comments?post=1570"}],"version-history":[{"count":1,"href":"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-json\/wp\/v2\/posts\/1570\/revisions"}],"predecessor-version":[{"id":1577,"href":"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-json\/wp\/v2\/posts\/1570\/revisions\/1577"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-json\/wp\/v2\/media\/1571"}],"wp:attachment":[{"href":"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-json\/wp\/v2\/media?parent=1570"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-json\/wp\/v2\/categories?post=1570"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/pohled.podhrazsky.eu\/subdom\/pohled\/wp-json\/wp\/v2\/tags?post=1570"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}